Next-Gen Flow Cytometry & EV Instrumentation 2024
Date: Monday, 2 December 2024 - Tuesday, 3 December 2024
Location: Oregon City/Portland, Oregon
Confirmed Speakers and Chairpersons
Daniel Chiu, A. Bruce Montgomery Professor of Chemistry, University of Washington -- Conference Co-Chairperson
Michael Graner, Professor, University of Colorado Anschutz Medical Campus
Stuart Ibsen, Associate Professor, The Knight Cancer Institute’s Cancer Early Detection Advanced Research Center (CEDAR), Oregon Health and Science University
Tijana Jovanovic-Talisman,
Associate Professor, Beckman Research Institute, City of Hope
Julie Saugstad, Professor, Dept of Anesthesiology & Perioperative Medicine Research Division, OHSU
Sarah Andres, Assistant Professor, School of Medicine, Dept of Pediatrics, OHSU
Sven Kreutel, CEO, Particle Metrix, Inc.
Yuguang Liu, Assistant Professor, Mayo Clinic
Mancy Tong, Assistant Professor, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of Medicine
Setty M. Magaña, Assistant Professor of Neurology & Pediatrics, Nationwide Children's Hospital
Terry Morgan, Professor of Pathology and Laboratory Medicine, Oregon Health and Science University -- Conference Co-Chairperson
SelectBIO Next-Gen Flow Cytometry and EV Instrumentation brings together researchers and industry participants from both academia and industry focusing on technology development and utilization for Next-Generation Flow Cytometers with Ability to Study EVs and EV Instrumentation that are driving research in the EV field.
Presentations at this conference focus upon technology development, new instrument platforms, applications, new biological investigations on EVs driven by these instrumentation platforms. Also new segments of research enabled by these innovations in instrumentation are presented at this conference.
Additionally, this is a hands-on demo conference enabling instrument demos onsite for researchers to view various instrument platforms in "live mode."
Conference is Composed of:
Scientific Presentations from Key Opinion Leaders in the Field
Technology Presentations from Companies Developing Technologies and Products for EV Research
Hands-on Demos with Instrumentation by the Potential End-Users
EV New Studies/Applications Enabled by New Instrumentation
Lots of Networking Sessions for Scientific Exchange
Call for Presentations and Posters
Agenda Topics
You can present your research in a talk or a poster while attending the meeting. Submit an abstract for consideration now!
Abstract Submission Deadline: 31 October 2024
Flow Cytometry Platforms for Studying Extracellular Vesicles (EVs)
Single EV Analysis
Biological Studies Possible Using Single EV Analysis
Nanoparticle Tracking (NT) and Its Utilization in EV Research
Advances in NT Technology
Emerging EV Research Areas
Sponsorship and Exhibition Opportunities
Jeff Fan
Exhibition Manager - SelectBIO
E-mail: Jeff@selectbioconferences.com
3 for 2 Offer on Delegate Registrations
SelectBIO are offering 3 Delegate Registrations for the price of 2 on all delegate passes. To take advantage of this offer, please contact us by email, phone or click the Contact Us button below. Looking for more than 3 Delegate Passes? Contact us for more information on our special rates for large groups.
Any questions or assistance during registration, please call us at: +1 (510) 857-4865 or e-mail us at: Contact SelectBIO
Gold Sponsors
Exhibitors
Sponsorship and Exhibition Opportunities
If you require any information about exhibiting or sponsoring at one of our events please contact Jeff Fan using the information below:
Jeff Fan
Exhibition Manager - SelectBIO
Email: Jeff@selectbioconferences.com
Why Exhibit at a SelectBIO Conference?
Specialists: SelectBIO doesn't organize conferences in shipping, accountancy, textiles etc. – just drug discovery and the life sciences. Many of our staff have bioscience qualifications and many years of experience. So, we speak your language and understand your needs.
Superior Customer Service: Our sales team will take care of you with specialist advice and customized packages. We don’t forget you after you sign on the bottom line either as our customer service dept. will alert you to all the things you need to think about up to and during the event itself.
Next-Gen Flow Cytometry and EV Instrumentation 2024 Conference Venue
SelectBIO is delighted to host the Next-Gen Flow Cytometry and EV Instrumentation in Oregon City, Oregon -- this is approximately 15-miles from Portland International Airport (PDX).
**Flying in: Please Fly into PDX Airport**
Conference Venue Address:
Museum of the Oregon Territory
211 Tumwater Drive
Oregon City, Oregon 97045
This beautiful conference venue overlooks the Willamette River Falls -- the Willamette River is one of only two rivers in the world that flows from South to North (the other one is the Nile).
Additionally this conference venue houses a museum detailing the history of Oregon as a Western State Involved in the Expansion of the USA -- view historical artifacts, an actual stage coach used by the settlers to come to the Oregon territory, and a courthouse setup just as the 1800s where land claims for settlers were heard. A truly remarkable illustration of history of this region and available for conference participants to view at no extra charge -- provides a perspective to Oregon and of times past which helped to build and shape this western frontier.
Hotel Bookings Can be Made at the Following Hotel (1.2 miles from the Conference Venue):
Best Western Plus River Shore Hotel
1900 Clackamette Drive
Oregon City, Oregon 97045
Pricing is US$124 per night (+taxes, fees extra)
This room block rate expires on November 1, 2024 so please book prior to that date to lock-in your room booking.
To make your Hotel Reservations Online:
For any hotel reservation-related issues, or if you need any help with hotel bookings, please contact:
Jeff Fan
Events Manager, SelectBIO
E-mail: Jeff@selectbioconferences.com
SelectBIO has NOT authorized ANY third party company to assist in hotel bookings or reservations for the conference. Please do NOT do business with any third party companies. If in doubt, please contact Jeff Fan immediately to clarify.
Register to this conference and also enjoy the following co-located events at no extra charge.
Training Courses
If you would like to submit a proposal for an oral or poster presentation at this meeting, please fill out the form below required for your submission.
Successful applicants will be provided with all necessary information.
Abstract Content
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Written in English
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Written in the third person
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Include title, name(s) and affiliation(s) of the authors
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Between 100 - 200 words
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Suitable for direct publication in the proceedings pack and on the website
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Related to the subject of the conference
Agenda Topics
Flow Cytometry Platforms for Studying Extracellular Vesicles (EVs)
Single EV Analysis
Biological Studies Possible Using Single EV Analysis
Nanoparticle Tracking (NT) and Its Utilization in EV Research
Advances in NT Technology
Emerging EV Research Areas
Copyrights
The presenting author/person who submitted the abstract assumes full responsibility of the content of the abstract and we assume that all co-authors are aware of this content. Please note that your biography, summary and abstract may be used on this website and conference materials.
Sarah Andres Biographical Sketch
Sarah Andres, Assistant Professor, School of Medicine, Dept of Pediatrics, OHSU
Dr. Sarah Andres is an Assistant Professor of Pediatrics at Oregon Health and Science University. She has a long-standing interest in translational research centered on cellular and molecular mechanisms of gastrointestinal homeostasis and disease. Her lab is interested in developing novel approaches to treat intestinal inflammation and barrier dysfunction. This includes studying the effects of human digestion on human milk extracellular vesicles and how these in turn impact intestinal physiology. Additionally, her team is studying post-transcriptional regulation via RNA binding proteins in the neonatal inflammatory disease, necrotizing enterocolitis. The Andres lab uses human patient samples, including intestinal organoid (enteroid) models, genetic mouse models, and molecular biology approaches to conduct their translational studies. Dr. Andres is a passionate mentor who loves to share her work and love of science with others, including trainees, other scientists, and the public.
Julie Saugstad Biographical Sketch
Julie Saugstad, Professor, Dept of Anesthesiology & Perioperative Medicine Research Division, OHSU
Dr. Julie Saugstad is a tenured Professor in the Department of Anesthesiology & Perioperative Medicine at the Oregon Health & Science University with joint appointments in Molecular & Medical Genetics, and Neurology. She earned her PhD in Biochemistry and Molecular Biology from the Oklahoma University Health Science University, then did a Postdoctoral Fellowship at the Vollum Institute at OHSU to gain expertise in molecular neurobiology. Funded by the first NIH Extracellular RNA Communication program, her OHSU team were the first to discover miRNA biomarkers for Alzheimer’s disease (AD) in cerebrospinal fluid (CSF) from living donors, and then validated these miRNA biomarkers in a new cohort of participants and identified miRNAs that trend with progression from control to mild cognitive impairment (MCI) to AD. A subsequent R01 established the utility of the AD CSF miRNAs as biomarkers in plasma, and the longitudinal stability of AD miRNAs in plasma. They are currently examining the miRNAs as early biomarkers for AD in patients diagnosed with MCI, and the specificity of the miRNAs for AD vs. related neurodegenerative disorders (Parkinson’s disease and Lewy body dementia). With a new R01 they are establishing biomarkers for AD based on the physical features of extracellular vesicles (EVs) from living donor human CSF, and have reported differential effects of APOE genotype on CSF EV miRNAs in females with AD compared to males. They are also using vesicle flow cytometry to measure the size and concentration of CSF EVs from AD and PD vs. controls, using multiplex bead-based flow cytometry to identify surface marker proteins altered in AD CSF EVs, and determining the expression of AD miRNAs and their target proteins in post-mortem human brain tissue sections.
Stuart Ibsen Biographical Sketch
Stuart Ibsen, Associate Professor, The Knight Cancer Institute’s Cancer Early Detection Advanced Research Center (CEDAR), Oregon Health and Science University
My research group’s overall scientific mission is to develop an understanding of the nanoparticles released by tumors into circulation. This includes the role that cancer phenotypes play in the production of these nanoparticles, how the nanoparticles interact with tissues throughout the body, and how these particles can be used clinically for cancer detection and screening. In order to answer these questions, my goal is to develop high conductance dielectrophoresis (DEP) techniques to recover cancer-derived nanoparticles straight from human plasma for the characterization of their cancer related biomarker payloads. In particular, my research has focused on characterizing their biomarker payloads through immunostaining and PCR based methods. My group brings a unique approach to the field of cancer liquid biopsy through the development of these DEP techniques that are capable of collecting multiple different nanoparticle types simultaneously from the same plasma sample. Our team is committed to enable the use of circulating nanoparticles for clinical diagnosis applications.
Michael Graner Biographical Sketch
Michael Graner, Professor, University of Colorado Anschutz Medical Campus
Michael Graner received his PhD in Biochemistry from the University of Illinois followed by post-doctoral and research faculty work at the University of Arizona, shifting gears from the Drosophila extracellular matrix to cancer immunotherapy. He then took at faculty position at Duke University’s Tisch Brain Tumor Center, followed by his current position as Professor in Neurosurgery at the University of Colorado Denver (Anschutz Medical Campus). He is also a member of the University of Colorado Cancer Center, the Colorado Clinical and Translational Sciences Institute, the MAVRC Program, and holds a Visiting Professorship Appointment at the Shenzhen Third People’s Hospital (China) and an adjunct faculty appointment at Colorado State University. Graner has a long-standing interest in cell stress responses, which led to cancer vaccine development (including one in clinical trials), which somehow led to the world of extracellular vesicles (EVs). His lab currently concentrates on signaling mechanisms involving EVs, in particular the transfer of stressed phenotypes from stressed tumor cells to unstressed ones via EVs.
Mancy Tong Biographical Sketch
Mancy Tong, Assistant Professor, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of Medicine
Dr Mancy Tong is an Assistant Professor in the Department of Obstetrics, Gynecology and Reproductive Sciences at Yale School of Medicine. She has had over a decade of research experience in furthering our understanding of how the semi-allogeneic placenta survives and thrives adjacent to the maternal decidua throughout pregnancy and the roles that extracellular vesicles play in mediating this process during both normal and pathological pregnancies. Her NIH-funded laboratory currently focusses on examining the impacts of early maternal viral exposure and resultant inflammation on the formation of the maternal decidua in preparation for implantation, and downstream effects on pregnancy success.
Setty M. Magaña Biographical Sketch
Setty M. Magaña, Assistant Professor of Neurology & Pediatrics, Nationwide Children's Hospital
Dr. Magaña is an attending physician-scientist and Co-Director of the Neuroimmunology Program at Nationwide Children’s Hospital and Assistant Professor of Clinical Pediatrics and Neurology at The Ohio State University College of Medicine. She completed her Clinical and Translational Sciences Ph.D. at Mayo Graduate School of Biomedical Sciences, her Pediatric Residency and Child Neurology Residency at Mayo Clinic College of Medicine, and a Neuroimmunology and Neuroinfectious Diseases fellowship at the National Institute of Neurological Disorders and Stroke. Dr. Magaña’s clinical and research interests are in understanding the disease mechanisms in pediatric and adult acquired demyelinating diseases, such as Multiple Sclerosis. She is affiliated with the International Pediatric MS Study Group, Acute Flaccid Myelitis Working Group and the International Society for Extracellular Vesicles CSF Task Force.
Yuguang Liu Biographical Sketch
Yuguang Liu, Assistant Professor, Mayo Clinic
Dr. Yuguang Liu is an Assistant Professor and Associate Consultant in the Department of Physiology and Biomedical Engineering and the Department of Immunology, as well as the Microbiome Program, Center for Individualized Medicine at Mayo Clinic. She is also affiliated with Trained as an electrical engineer, Yuguang Liu’s research interests focus on developing microfluidic technologies for various applications, from basic and translational research in medicine to the exploration of the limits of life on extraterrestrial bodies. She has expertise in developing microfluidic devices for studying the genomic adaption of single microbial cells under unusual environments, interaction between immune and microbial cells, rapid bacterial diagnosis and monitoring immune responsiveness to cancer immunotherapies. Yuguang Liu is also a recent awardee of Maximizing Investigators' Research Award (MIRA) R35 from the National Institute of General Medical Sciences.
Tijana Jovanovic-Talisman Biographical Sketch
Tijana Jovanovic-Talisman, Associate Professor, Beckman Research Institute, City of Hope
Dr. Jovanovic-Talisman received her B.Sc. in Physical Chemistry from the University of Belgrade, Serbia and Ph.D. in Chemistry from Columbia University, New York. She subsequently pursued postdoctoral fellowships at the Rockefeller University, New York and at the National Institutes of Health, Maryland. After two years as an Assistant Professor of Chemistry at the University of Hawaii at Manoa, she joined the Department of Cancer Biology and Molecular Medicine at the Beckman Research Institute of City of Hope, California where she is currently an Associate Professor. Her laboratory advances new methods to image single extracellular vesicles with quantitative microscopy.
Sven Kreutel Biographical Sketch
Sven Kreutel, CEO, Particle Metrix, Inc.
Sven Kreutel studied biology at the University of Hohenheim in Stuttgart, Germany. After his graduation in biology, he received his PhD in microbiology and protein biochemistry at the University of Hohenheim under Prof. Dr. Andreas Kuhn and Dr. Dorothee Kiefer working on the light sensing and signal transduction in photosynthetic bacteria. Since then, he worked in different sales and sales manager positions within the life science industry from clinical research over biology to nanoparticle sciences.
Daniel Chiu Biographical Sketch
Daniel Chiu, A. Bruce Montgomery Professor of Chemistry, University of Washington
Daniel T. Chiu is currently the A. Bruce Montgomery Professor Chemistry, Endowed Professor of Analytical Chemistry, and Professor of Bioengineering at the University of Washington. He is a member of the University of Washington’s Center for Nanotechnology, Neurobiology and Behavior Program, and the Cancer Consortium of the Fred Hutchinson Cancer Research Center. He has authored more than 180 publications and is the inventor on over 40 issued patents. Dr. Chiu obtained a B.A. in neurobiology and a B.S. in chemistry at the University of California, Berkeley in 1993, and a Ph.D. in chemistry from Stanford University in 1998.
Terry Morgan Biographical Sketch
Terry Morgan, Professor of Pathology and Laboratory Medicine, Oregon Health and Science University
Dr. Morgan is a Professor of Pathology and Biomedical Engineering at Oregon Health & Science University (OHSU) in Portland, Oregon, USA. He has been an NIH-funded investigator since 2012 and he has worked with BD Biosciences since 2016 to develop, validate, and begin research trials using nanoscale high resolution flow cytometry to image, count, and isolate extracellular vesicles (EVs). Dr. Morgan is the leader of the EV research group at the Center for Developmental Health at OHSU. He is also the leader of the flow cytometry EV group at the Knight Cancer Institute to test its potential as a diagnostic platform for early cancer detection.
08:00
2 December 2024
Conference Registration
Conference Registration, Materials Pick-Up, Coffee, Tea, Pastries and Networking
09:00
2 December 2024
Tumwater Ballroom
Terry Morgan, Professor, Oregon Health & Science University (OHSU), United States of America -- Conference Co-Chairperson
Welcome and Introduction
09:10
2 December 2024
Tumwater Ballroom
Keynote Presentation
Daniel Chiu, A. Bruce Montgomery Professor Chemistry, Endowed Professor of Analytical Chemistry, and Professor of Bioengineering at the University of Washington, United States of America -- Conference Co-Chairperson
Technological Landscape of Single-EV Analysis
Extracellular vesicles (EVs) are highly heterogeneous and comprise a diverse set of surface protein markers as well as intra-vesicular cargoes. To fully understand the heterogeneity of EVs and to identify the relevant EV subpopulations of interest, it is important to study EVs using combination biomarkers with single-EV sensitive techniques. Here, I describe and discuss the current state-of-the-art in single-EV analysis, with emphasis on flow and imaging based approaches.
09:45
2 December 2024
Tumwater Ballroom
Keynote Presentation
Michael Graner, Professor, University of Colorado Anschutz Medical Campus, United States of America
Extracellular Vesicles and Particles in Disease or as Cures: A Brief Example of Fibrosis
Research on extracellular vesicles (EVs) and particles (EPs) has exploded in recent years. Originally seen as curiosities found outside of cells and in blood or other biofluids, these entities are now the subject of intense study in nearly every biologic system and almost any disease we know of. Areas of research range from basic biology (fundamental origins of EV-Ps), to cargo characterization of EV-Ps, to their effects as purveyors of either pathologies or therapies. This talk will highlight examples of EV-Ps driving pathology, with a focus on glial scarring and fibrosis, and then delve into therapeutic strategies involving EV-Ps with an eye towards both their pros and cons.
10:20
2 December 2024
Tumwater Ballroom
Morning Coffee Break and Networking
11:00
2 December 2024
Tumwater Ballroom
Tijana Jovanovic-Talisman, Associate Professor, Beckman Research Institute, City of Hope, United States of America
SEVEN O’Clock: Time for a New Method to Characterize Individual Extracellular Particles
Extracellular vesicles (EVs) are membrane-encapsulated nanoparticles shed from all cells. In addition to their critical roles in cellular functions, EVs are emerging as exciting therapeutic delivery vectors and biomarker candidates. Our group has recently developed Single Extracellular VEsicle Nanoscopy (SEVEN), which combines affinity isolation of EVs with super-resolution microscopy to enable a multiparametric characterization of EVs. With ~7 nm precision and molecular sensitivity, this method can robustly provide critical parameters of individual EVs: concentration, size, shape, molecular cargo content, and heterogeneity. We show that SEVEN can specifically detect rare EV populations, including tissue-associated and disease-associated EVs. Because it attains nanoscale precision, SEVEN can also detect small non-vesicular extracellular nanoparticles. Importantly, EV studies may be democratized via a new method that employs super-resolution microscopy and advanced algorithms supported by machine learning-based EV assessment. Our approach – which we termed SEVEN-universal protocol (SEVEN-UP) – can robustly assess EV concentration, size, and molecular content. Altogether, SEVEN and SEVEN-UP may be important tools for significantly propelling our knowledge-base of EVs forward.
11:30
2 December 2024
Tumwater Ballroom
Sven Kreutel, CEO, Particle Metrix, Inc., United States of America
Characterization of Extracellular Vesicles and Other Biological Nanoparticles using Nanoparticle Tracking Analysis (NTA)
Nanoparticle Tracking Analysis (NTA) has emerged as a fast and vital characterization technology for Extracellular Vesicles (EVs), Exosomes and other biological material in the size range from 30 nm to 1 μm. While classic NTA scatter operation feeds back the size and total particle concentration, the user typically cannot discriminate whether the particle is a vesicle, protein aggregate, cellular trash or an inorganic precipitate. The fluorescence detection capabilities of f-NTA however enables the user to gain specific biochemical information for phenotyping of all kinds of vesicles and viruses. Alignment-free switching between excitation wavelengths and measurement modes (scatter and fluorescence) allow quantification of biomarker ratios such as the tetraspanins (CD63, CD81 and CD9) within minutes. Furthermore, specific colocalization studies using c-NTA gives a deeper understanding of the composition of biomarker on single particle.
12:00
2 December 2024
Tumwater Ballroom
Yuguang Liu, Assistant Professor, Mayo Clinic, United States of America
A Digital Microfluidic Platform for Rapid and Automated EV Extraction and Detection
Extracellular vesicles (EV) have been gaining attention as a promising biomarker for cancer diagnostics and therapeutic monitoring. For example, in recipients of PD1/PD-L1 immune checkpoint inhibitors – a key pillar of cancer immunotherapy, EV-derived PD-L1 molecules are increasingly recognized as a non-invasive key indicator of therapeutic responsiveness. Conventional methods to measure EV-derived PD-L1 molecules requires ultracentrifugation to separate EVs and quantify those that express PD-L1 using nanoscale flow cytometry. These are tedious processes that require trained technologists in centralized facilities, which is a key barrier to the close monitoring of these EV-based indicators of therapeutic responsiveness. However, frequently evaluating the immune responsiveness to the immunotherapies is essential, because prolonged use of a futile therapy can lead to the loss of time in the race with the fatal disease, as well as financial burden on patients, healthcare systems and economy. To bridge the gap, we develop a proof-of-concept microfluidic device that can separate EVs directly from biofluids and quantify PD-L1-positive EVs automatedly. In this device, EVs can be extracted by immunomagnetic beads, and the PD-L1 expression level can be quantified with the electrochemical sensor on-chip < 2 hours. Meanwhile, the device can enable 10X increase in the number of EVs extracted compared with in-tube extraction, with the ability to distinguish 10X differences in EV concentration. This tool can ultimately help to identify futile therapies quickly and prompt personalized alternatives, as well as to collect densely time-longitudinal data points so that we can extrapolate future response trajectories and thus inform strategies to curve the outcome.
12:30
2 December 2024
Tumwater Ballroom
Setty M. Magaña, Assistant Professor of Neurology & Pediatrics, Nationwide Children's Hospital, United States of America
Leveraging Multiparametric Bulk and Single EV Methods for Biomarker Discovery in Obesity-Associated Pediatric Neuroimmune Diseases
Multiple Sclerosis is a chronic inflammatory demyelinating disorder of the central nervous system (CNS). Pediatric-onset MS represents 3-10% of all MS cases. The cause of MS is unknown, however it is driven by a complex interplay of genetic and environmental factors. Interestingly, childhood obesity has been shown to be an independent risk factor for MS but the underlying cellular and molecular mechanisms remain enigmatic. Extracellular vesicles (EVs) have gained increased attention as novel orchestrators of intercellular communication and key mediators of CNS neuroimmune cross-talk and adipose-tissue metabolic cross-talk. We present a systematic approach for establishing a tissue-specific EV signature which can then be applied to circulating biomarker discovery.
13:00
2 December 2024
Tumwater Ballroom
Networking Lunch
14:20
2 December 2024
Tumwater Ballroom
Keynote Presentation
Terry Morgan, Professor of Pathology, Obstetrics & Gynecology, and Biomedical Engineering, OHSU, United States of America
NextGen High Yield NanoFACS
Extracellular vesicles include a range of submicron particles, including small exosomes derived from endosomal biogenesis, larger microvesicles that bud directly from the cell membrane, and fragments of cells undergoing necrosis and apoptosis. These lipid-encapsulated EVs contain a variety of RNA species (including microRNAs) that provide insights into their cell source and potential function. Methods like size exclusion chromatography (SEC) provide a rich heterogeneous mixture of EVs from a variety of cell sources, and in a variety of sizes, but it lacks cell-specificity. Affinity capture lacks size-specificity, which is important because larger microvesicles have entirely different cargo than small EVs and larger EV contents are present in exponentially greater amounts than EVs with smaller diameters. In collaboration with BD Biosciences, we have developed a multiplex nanoscale high resolution flow cytometry (nanoFACS) platform to image, count, and isolate EVs. We can flow sort cell- and size-specific EVs and compare their contents with 100nm liposomes spiked into the same biofluid to control for background contamination inherent in any other EV isolation method. We have also developed a customized 50um nanoFACS flow sorting nozzle with protocol refinements that significantly advanced our methodological capabilities to isolate cell- and size-specific EVs more efficiently for cargo and functional analyses.
15:00
2 December 2024
Tumwater Ballroom
Mancy Tong, Assistant Professor, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of Medicine, United States of America
Extracellular Vesicles as Key Mediators of Bidirectional Maternal-Placental Crosstalk
Viral infections and resultant inflammation are major risk factors for miscarriage and later obstetrical complications including preeclampsia and intrauterine growth restriction. The Tong lab is interested in examining the impact of virus-induced inflammation on the preparation of the maternal endometrium for implantation and the roles that endometrial extracellular vesicles may play in dictating early trophoblast function and subsequent pregnancy success.
15:30
2 December 2024
Tumwater Ballroom
Mid-Afternoon Coffee Break
16:00
2 December 2024
Tumwater Ballroom
Hands-On EV Instrumentation Demo Session -- Particle Metrix
17:00
2 December 2024
Tumwater Ballroom
Networking Reception with Beer and Wine
18:00
2 December 2024
Tumwater Ballroom
Close of Day 1 of the Event
08:00
3 December 2024
Tumwater Ballroom
Morning Coffee, Tea, Pastries and Networking
09:00
3 December 2024
Tumwater Ballroom
Stuart Ibsen, Associate Professor, The Knight Cancer Institute’s Cancer Early Detection Advanced Research Center (CEDAR), Oregon Health and Science University, United States of America
Pancreatic Cancer Detection using Proteins, Cell-Free DNA, and Protease Activity Biomarkers Associated with Extracellular Vesicle Nanoparticles Collected Using Dielectrophoresis
Cancer-derived extracellular vesicle (EV) nanoparticles released into circulation carry with them a wide range of important biomarkers. One of the main challenges of using them for clinical diagnostic applications is the difficulty of recovering them from plasma, which is a critical step for biomarker quantification. Here we demonstrate the use of dielectrophoresis (DEP) microelectrode array chip technology to recover these nanoparticles directly from plasma in a label free way that relies on the dielectric properties of the EVs as whole particles rather than on specific biochemical characteristics. Furthermore, we demonstrate the ability to analyze several different classes of biomarkers directly on the chip after EV collection and purification. This includes the overall concentration of cell-free DNA with its cancer-related mutations, protein biomarkers that are over-expressed by the tumor, and protease activity associated with the nanoparticles. We demonstrate the efficacy of this diagnostic technique through the combination of the cell-free DNA and the protein biomarkers to successfully differentiate patients with pancreatic cancer from patients with benign pancreatic disease. Using a support vector machine learning algorithm analysis, we show that the AUC of this DEP-based technique is 0.93 which increases to 0.97 when applied to patients over the age of 50. This is compared to the standard invasive endoscopic ultrasound-guided fine needle aspiration diagnostic procedure that has an AUC of 0.79. We show that protease activity on the DEP isolated EVs can be used to successfully different between patients with pancreatic cancer and patients with benign pancreatic cysts. We also show the collection of cf-DNA nanoparticles that contain mutant KRAS fragments. Together, the ability to quickly and efficiently recover EV nanoparticles directly from plasma and analyze biomarker content directly on the chip demonstrates the necessary characteristics for translation to the clinical laboratory setting.
09:30
3 December 2024
Tumwater Ballroom
Hands-On EV Instrumentation Demo Session -- BD Biosciences
10:30
3 December 2024
Tumwater Ballroom
Mid-Morning Coffee Break and Networking
11:00
3 December 2024
Tumwater Ballroom
Julie Saugstad, Professor, Dept of Anesthesiology & Perioperative Medicine Research Division, OHSU, United States of America
MicroRNAs as Biomarkers for and Mediators of Alzheimer’s Disease
This talk will present data to support that microRNAs in cerebrospinal fluid from living donors can be used as biomarkers for Alzheimer’s disease.
11:30
3 December 2024
Tumwater Ballroom
Round-Table Discussion: Trends in EV Research and Unmet Needs in Instruments and Reagents
Chaired by Professor Terry Morgan and Professor Daniel Chiu
12:00
3 December 2024
Tumwater Ballroom
Networking Lunch
13:00
3 December 2024
Tumwater Ballroom
Demo Session -- Cytek Biosciences
14:00
3 December 2024
Tumwater Ballroom
Sarah Andres, Assistant Professor, School of Medicine, Dept of Pediatrics, OHSU, United States of America
Digestive Fluid Extracellular Vesicles of Milk-Fed Infants are Absorbed by Neonatal Intestinal Epithelial Cells
This talk will present data to support that extracellular vesicles can be isolated from small volumes of fed- and digested human milk and show that isolated vesicles are absorbed by primary neonatal epithelial cells via endocytosis.
14:30
3 December 2024
Tumwater Ballroom
Mid-Afternoon Coffee Break